Reference type: Journal
Authors: Shah KA, Halquist MS, Karnes HT
Article Title: A modified method for the determination of tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human urine by solid phase extraction using a molecularly imprinted polymer and liquid chromatography tandem mass spectrometry.
Publication date: 2009
Journal: Journal of Chromatography B
Volume: 877
Issue: (14-15)
Page numbers: 1575-1582.
DOI: 10.1016/j.jchromb.2009.04.003
Alternative URL: http://www.sciencedirect.com/science/article/B6X0P-4W15KTP-1/2/5cc73d82fd78ad346dc5d93752d8beea

Abstract: The work described in this paper represents an improvement over a previously published method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in human urine by solid phase extraction on a molecularly imprinted polymer column coupled with HPLC and -MS/MS detection. The influence of ion suppression due to sample matrix effect was evaluated, and found to influence the response of NNAL. By changing the liquid chromatography conditions, the response for this method was enhanced approximately 25-fold through avoidance of ionization suppression that was found with a previously published method and sample throughput has been improved. The dynamic range of the assay extends from 20 to 2500ápg/mL with a mean r2á>á0.998. The lower limit of quantitation for the assay was 20ápg/mL despite the use of an inherently lower sensitivity instrument. The method was validated according to current FDA guidelines for bioanalytical method validations
Template and target information: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, NNAL
Author keywords: Tobacco specific nitrosamine, 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol, NNAL, molecularly imprinted polymer, Matrix effect, LC-MS, MS