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Reference type: Journal
Authors: Knopp D, Zhu QZ, Carrasco PB, Sire SG, Haupt K, Schedl M, Scharnweber T, Bayona JM, Niessner R
Article Title: Comparison of molecularly imprinted polymers (MIPs) and sol-gel based immunoaffinity supports for the extraction of sulfonylurea herbicides from aqueous samples.
Publication date: 2001
Journal: Abstracts of Papers of the American Chemical Society
Volume: 221
Issue: (AGRO)
Page numbers: 61.

Abstract: No data are available on the use of antibodies or molecularly imprinted polymers (MIPS) for a more selective isolation (immunoextraction) of sulfonyl urea herbicides from real samples. Molecular imprinting is a relatively new technique which allows the formation of selective recognition in a stable polymer matrix. In this method, polymerizable functional monomers are prearranged around a template molecule by non-covalent or covalent interactions prior to initiation of polymerization. Preparation and characterization of MIPs for metsulfuron-methyl will be demonstrated. lmmunoaffinity chromatography uses the highly specific antigen-antibody interactions for the selective separation of antigens from complex mixtures. In this project, using polyclonal antibodies for metsulfuronmethyl, the immunoadsorbents were prepared by the sol-gel method which is a mild technique to immobilize biomolecules in the pores of a hydrophilic glass matrix. In this presentation, first results will be discussed on the selective extraction of metsulfuron-methyl from aqueous samples using both MIPs and the immunosorbent


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