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Reference type: Journal
Authors: Diltemiz SE, Hür D, Ersöz A, Denizli A, Say R
Article Title: Designing of MIP based QCM sensor having thymine recognition sites based on biomimicking DNA approach.
Publication date: 2009
Journal: Biosensors and Bioelectronics
Volume: 25
Issue: (3)
Page numbers: 599-603.
DOI: 10.1016/j.bios.2009.01.032
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4VHGC26-2/2/8fc50d84748b8ac0b348d3858a4bbb51

Abstract: Quartz crystal microbalance (QCM) sensors coated with molecular imprinted polymers (MIP) have been developed for the determination of thymine. In this method, methacryloylamidoadenine (MA-Ade) have used as a new monomer and thymine template for inspiration of DNA nucleobases interaction. The thymine can be simultaneously hydrogen binding to MA-Ade and fit into the shape-selective cavities. Thus, the interaction between nucleobases has an effect on the binding ability of the QCM sensors. The binding affinity of the thymine imprinted sensors has investigated by using the Langmuir isotherm. The thymine imprinted QCM electrodes have shown homogeneous binding sites for thymine (Ka: 1.0áÎá105áM-1) while heterogeneous binding sites for uracil. On the other hand, recognition selectivity of the QCM sensor based on thymine imprinted polymer toward to uracil, ssDNA and ssRNA has been reported in this work
Template and target information: thymine
Author keywords: Molecular imprinting polymers (MIP), quartz crystal microbalance (QCM), thymine, DNA, Thymine imprinted sensors


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