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Reference type: Journal
Authors: Rejtharová M, Rejthar L
Article Title: Determination of chloramphenicol in urine, feed water, milk and honey samples using molecular imprinted polymer clean-up.
Publication date: 2009
Journal: Journal of Chromatography A
Volume: 1216
Issue: (46)
Page numbers: 8246-8253.
DOI: 10.1016/j.chroma.2009.07.037
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4WV15R4-1/2/061bd86162db6b48e9847a58fd3c269f

Abstract: A method for determination of low concentrations of chloramphenicol in urine, feed water, milk and honey was developed. A comparison was carried out between a routinely used analytical method based on solid phase extraction (SPE-C18) for cleaning the extract and the new procedure for the sample preparation using columns based on the molecular imprinted polymers (MIP) principle. The extracts obtained from the MIP clean-up procedure were clean enough for chromatografic analyses. Confirmatory analyses were conducted using GC/MS-NCI after derivatisation (silylation). The described method was fully validated according to CD 2002/657/EC. This method is considerably robust and allows very dirty samples to be processed. The described MIP procedure is very simple and low-time-consuming, and provides high throughput of the samples examined. This could be used for routine screening and confirmatory analyses as well
Template and target information: chloramphenicol
Author keywords: molecular imprinted polymer (MIP), chloramphenicol, Gas chromatography-mass spectrometry-negative chemical ionization (GC/ MS-NCI)


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