Abstract: Determination of cyanide or its metabolites in biological fluids is necessary for forensic, clinical, military, research, and veterinary purposes. Methods of directly evaluating cyanide levels are limited by the volatility of cyanide, and by the difficulty of establishing steady-state cyanide levels with time. These studies focus on developing a new analytical technique to determine the chemically stable urinary metabolite of cyanide, 2-aminothiazoline-4-carboxylic acid (ATCA), in mice liver samples. Two extraction techniques, solid phase extraction (SPE) and molecular imprinted polymer stir bar (MIP-SB), were tested to determine the efficiency of ATCA extraction. Mice were exposed to various doses of cyanide. A method was also developed to dissect, preserve organs, and homogenize the livers. The new analytical method development of molecularly imprinted polymers (MIPs) on the surface of a silica cylinder serves as a selective molecularly imprinted stir bar sorption extraction (MISBSE) was previously reported. Using external calibration, the capacity of one MISBSE for ATCA was estimated about 31 ng. The MISBSE improved the ability to extract lower concentrations of ATCA. This new strategy of using MISBSE-ESI/MS/MS enhanced the selectivity and sensitivity for low concentration of ACTA from organ-samples after cyanide exposure. Ongoing studies are focusing on developing alternative methods, e.g. surface enhanced Raman sensors to determine ATCA. Two effective sample preparation methods (SPE vs. MIP-SB) to extract ATCA from mice liver samples will be discussed and compared. The effectiveness of the extraction techniques are determined by employing known concentrations of ATCA evaluated by the LC/MS/MS. Liver ATCA contents are compared to the dose of cyanide mice were given.
Template and target information: 2-aminothiazoline-4-carboxylic acid, ATCA