MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Jackson R, Petrikovics I, Lai EPC, Yu JCC
Article Title: Molecularly imprinted polymer stir bar sorption extraction and electrospray ionization tandem mass spectrometry for determination of 2-aminothiazoline-4-carboxylic acid as a marker for cyanide exposure in forensic urine analysis.
Publication date: 2010
Journal: Analytical Methods
Volume: 2
Issue: (5)
Page numbers: 552-557.
DOI: 10.1039/b9ay00251k

Abstract: In forensic casework, a stable and quantifiable marker is desirable for the determination of cyanide poisoning in biological fluids. 2-Aminothiazoline-4-carboxylic acid (ATCA) is a chemically stable urinary metabolite of cyanide that has been considered to be a reliable biological marker for cyanide exposure. However, endogenous ATCA is always present in low quantity originating from either dietary intake of cyanide or from normal metabolism of amino acids. A selective and sensitive analytical method is needed to determine the endogenous level of ATCA in order to identify cyanide poisoning. The objective of this research was to prepare molecularly imprinted polymers (MIPs) on the surface of a silica stir bar for molecularly imprinted stir bar sorption extraction (MISBSE). Under optimal extraction conditions, the MISBSE could selectively preconcentrate ATCA from urine samples. The binding capacity of one MISBSE stir bar for ATCA was determined to be 35 3 ng (n = 3). Combining MISBSE with electrospray ionization tandem mass spectrometry (ESI/MS/MS), ATCA was detected without derivatization at the 400 ng/mL concentration level. This new strategy of MISBSE-ESI/MS/MS enhanced the selectivity and sensitivity for the detection of ACTA in urine samples.
Template and target information: 2-aminothiazoline-4-carboxylic acid, ATCA


  Periodic table Trainer shirt  Mug featuring the name Edward spelled out in the single letter amino acid code  Gravity waves shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner