MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Tse Sum Bui B, Merlier F, Haupt K
Article Title: Toward the Use of a Molecularly Imprinted Polymer in Doping Analysis: Selective Preconcentration and Analysis of Testosterone and Epitestosterone in Human Urine.
Publication date: 2010
Journal: Analytical Chemistry
Volume: 82
Issue: (11)
Page numbers: 4420-4427.
DOI: 10.1021/ac100128c

Abstract: A molecularly imprinted polymer (MIP), templated with methyltestosterone, has been synthesized for the cleanup of hydrolyzed urine samples for subsequent testosterone (T) quantification by LC-MS/MS. A concentration of 2 ng/mL testosterone could be quantified after a single step extraction on the MIP. The limit of detection and quantification with the criteria of a signal-to-noise ratio of 3 and 5 were 0.3 and 2 ng/mL, respectively. These values meet the conditions set by the World Anti-Doping Agency for the minimum required performance limits for doping controls, between 2 and 10 ng/mL. Epitestosterone (E) was also separated on this polymer and could be detected at concentrations down to 0.3 ng/mL. The quantification of T and E gives access to the determination of the T/E ratio, essential in doping analysis. Hence, our polymers can offer a more specific extraction procedure, resulting in increased sensitivity with limits of detection 10 times lower than the ones achieved by the standard SPE C18 sorbents employed in official testing laboratories
Template and target information: methyltestorsterone, testosterone, epitestosterone


  Shirt featuring the name Karsten spelled out in symbols of the chemical elements  Mug featuring the name Karsten spelled out in the single letter amino acid code  Perpetual student shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner