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Abstract: Molecular imprinted polymeric microspheres (MIPMs) were prepared by a one-step swelling polymerization method in an aqueous system using polystyrene microspheres as seeds and Gatifloxacin as template molecules. The recognition mechanism of MIPMs was evaluated by ultraviolet spectrometry. The infrared spectrum of the polymer further demonstrated that there were some functional groups in the MIPMs which could interact with the template molecules. The morphology of the MIPMs was measured by scanning electron microscopy. The binding characteristics were investigated by fluorescence spectrum. Scatchard analysis suggested that there were two classes of binding sites during MIPMs recognition of Gatifloxacin. Equilibrium dissociation constant K_D1 and maximum number B_max1 of binding site with high affinity were calculated to be 0.603 mmol/L and 28.62 μmol/g, while K_D2 and B _max2 of binding site with low affinity were 3.110 mmol/L and 74.27 μmol/g, respectively
Template and target information: gatifloxicin
Author keywords: Antibiotics pollution, Binding site, Gatifloxacin, Molecular imprinted, Scatchard analysis