MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Yu JH, Wan FW, Zhang CC, Yan M, Zhang XN, Wang SW
Article Title: Molecularly imprinted polymeric microspheres for determination of bovine serum albumin based on flow injection chemiluminescence sensor.
Publication date: 2010
Journal: Biosensors and Bioelectronics
Volume: 26
Issue: (2)
Page numbers: 632-637.
DOI: 10.1016/j.bios.2010.07.009
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-50HP2R9-1/2/02e9f5a60266eb98ceff79132d4dd363

Abstract: A novel flow injection chemiluminescence (FI-CL) sensor for the determination of bovine serum albumin (BSA) using molecularly imprinted polymeric microspheres (MIPMs) as recognition element is reported. The BSA-MIPM was synthesized by suspension polymerization in toluene, using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker in the bovine serum albumin template molecule. Scanning electron microscope (SEM) was employed to characterize the surface morphology of the resultant imprinted microspheres. Molecular modeling was employed to simulate the possible recognition process of the MIPM. Then the synthesized BSA-MIPM was employed as recognition element by packing into flow cell to establish a novel FI-CL sensor. The chemiluminescence (CL) intensity was correlated linear with the concentration of BSA over the range of 1.0 x 10-8 to 5.0 x 10-6 g mL-1 and the detection limit was 1.5 x 10-9 g mL-1. The relative standard deviation (RSD) for the determination of 1.0 x 10-7 g mL-1 BSA was 1.4% (n = 11). The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the new MIPM-CL sensor had been successfully applied to the determination of BSA in milk samples
Template and target information: protein, bovine serum albumin, BSA
Author keywords: chemiluminescence, Molecularly imprinted polymeric microspheres (MIPMs), flow injection, Bovine serum albumin (BSA)

  Periodic table Cream Cakes - the chemical formula for naughty but nice apron  Periodic table Bag bag  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner