MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Jing T, Du HR, Dai Q, Xia HA, Niu JW, Hao QL, Mei SR, Zhou YK
Article Title: Magnetic molecularly imprinted nanoparticles for recognition of lysozyme.
Publication date: 2010
Journal: Biosensors and Bioelectronics
Volume: 26
Issue: (2)
Page numbers: 301-306.
DOI: 10.1016/j.bios.2010.08.044
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-50THX92-K/2/b1a819b318d1413cd8603d68bbfbbe1b

Abstract: Molecular imprinting is an attractive technique for preparing mimics of natural, biological receptors. Nevertheless, the imprinting of macromolecule remains a challenge due to their bulkiness and sensitivity to denaturation. In this work, we presented a method for preparing multifunctional lysozyme-imprinted nanoparticles (magnetic susceptibility, molecular recognition and environmental response). The magnetic susceptibility was imparted through the successful encapsulation of Fe3O4 nanoparticles. Selective lysozyme recognition depended on molecularly imprinted film. Moreover, it was also a hydrophilic stimuli-responsive polymer, which could undergo a reversible change of imprinted cavity in response to a small change in the environmental conditions. Thus, magnetic molecularly imprinted nanoparticles had high adsorption capacity (0.11 mg mg-1), controlled selectivity and direct magnetic separation (22.1emu g-1) in crude samples. After preconcentration and purification with magnetic MIPs nanoparticles, a sensitive chemiluminescence method was developed for determination of lysozyme in human serum samples. The results indicated that the spiked recoveries were changed from 92.5 to 113.7%, and the RSD was lower than 11.8%
Template and target information: protein, lysozyme
Author keywords: molecular recognition, Magnetic susceptibility, surface imprinting, Environmental response, lysozyme

  mipdatabase.com logo imprinters do it in bulk shirt  Science Teacher peptide mug    Shirt featuring the template Lysozyme

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner