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Reference type: Journal
Authors: Rezaei B, Jafari MT, Rahmanian O
Article Title: Selective pretreatment and determination of phenazopyridine using an imprinted polymer-electrospray ionization ion mobility spectrometry system.
Publication date: 2011
Journal: Talanta
Volume: 83
Issue: (3)
Page numbers: 765-769.
DOI: 10.1016/j.talanta.2010.10.041
Alternative URL: http://www.sciencedirect.com/science/article/B6THP-51CJ373-B/2/47935ea5fcab29fa3e54ee62de190704

Abstract: In this research, selective separation and determination of phenazopyridine (PAP) is demonstrated using molecular imprinted polymer (MIP) coupled with electrospray ionization ion mobility spectrometry (ESI-IMS). In the non-covalent approach, selective MIP produced using PAP and methacrylic acid (MAA) as a template molecule and monomer, respectively. The created polymer is utilized as a media for solid-phase extraction (SPE), revealing selective binding properties for the analyte from pharmaceutical and serum samples. A coupled MIP-IMS makes it possible to quantitize PAP in the range of 1-100 ng mL-1 and with a 0.2 ng mL-1 detection limit. Furthermore, the MIP selectivity is evaluated by application of some substances with analogous and different molecular structures to that of PAP. This method is successfully applied for the determination of PAP in pharmaceutical and serum samples
Template and target information: phenazopyridine, PAP, 2,6-diamino-3-(phenylazo)pyridine
Author keywords: Phenazopyridine, molecularly imprinted polymer, Solid-phase extraction, ion mobility spectrometry

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