Authors: Shi XZ
, Song SQ
, Sun AL
, Li DX
, Wu AB
, Zhang DB
Determination of Chloramphenicol Residues in Foods by ELISA and LC-MS/MS Coupled with Molecularly Imprinted Solid Phase Extraction.
Publication date: 2010
Journal: Analytical Letters
Alternative URL: http://www.informaworld.com/10.1080/00032711003763616
Abstract: The combination of molecularly imprinted solid-phase extraction (MISPE) with ELISA and LC-MS/MS was developed for the detection of chloramphenicol (CAP) in honey samples. Significant recoveries of 99.1 ± 7.1 and 98.8 ± 8.2% were obtained for intra- and inter-assay determination by ELISA determination, respectively. The limit of detection of CAP was 0.034 μg kg-1 and the limit of quantification was 0.046 μg kg-1. Determination and validation of CAP by using LC-MS/MS were performed following the same extraction and purification process as for the ELISA. The results demonstrated that the CAP samples purified by using MISPE were simultaneously applicable to analysis by ELISA and LC-MS/MS
Template and target information: chloramphenicol, CAP
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