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Reference type: Journal
Authors: Qiao JD, Yan HY, Wang H, Wu YP, Pan PY, Geng YR
Article Title: Simultaneous Determination of Four Sudan Dyes in Egg Yolks by Molecularly Imprinted SPE Coupled with LC-UV Detection.
Publication date: 2011
Journal: Chromatographia
Volume: 73
Issue: (3)
Page numbers: 227-233.
DOI: 10.1007/s10337-010-1872-7

Abstract: A highly selective molecularly imprinted solid-phase extraction (MISPE) combined with liquid chromatography-ultraviolet detection was developed for the simultaneous isolation and determination of four Sudan dyes (I, II, III and IV) in egg-yolk products. The imprinted microspheres synthesized by suspension polymerization using phenylamine-naphthol as mimic template show high selectivity and affinity to the four kinds of Sudan dyes and were successfully applied as selective sorbents of solid-phase extraction for the simultaneous determination of the four Sudans from egg-yolk samples. Good linearity was obtained in a range of 0.062-10 μg g-1 and the average recoveries of the four Sudans at three spiked levels ranged from 94.1 to 102.5% with the relative standard deviations less than 5.8%. The developed extraction protocol eliminated the effect of template leakage on quantitative analysis and could be applied for the determination of Sudans in complicated food samples
Template and target information: Sudan dyes, Sudan I, Sudan II, Sudan III, Sudan IV
Author keywords: column liquid chromatography, molecularly imprinted solid-phase extraction, Imprinted microspheres, Sudan dyes, Egg yolk


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