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Reference type: Journal
Authors: Xu ZX, Gao HJ, Zhang LM, Chen XQ, Qiao XG
Article Title: The Biomimetic Immunoassay Based on Molecularly Imprinted Polymer: A Comprehensive Review of Recent Progress and Future Prospects.
Publication date: 2011
Journal: Journal of Food Science
Volume: 76
Issue: (2)
Page numbers: R69-R75.
DOI: 10.1111/j.1750-3841.2010.02020.x

Abstract: Abstract: Immunoassay, based on a selective affinity of the biological antibody for its antigen, is one of the most usual analytical methods in food safety and environmental chemistry. However, it presents several drawbacks because of the nature of the antibody. Molecular imprinting technique, due to its high selectivity and stability, ease of preparation and low cost, has shown great potential in producing artificial antibodies in biomimetic immunoassays. This article focuses on the recent states, advantages, current problems and outlooks of molecularly imprinted radio, fluoro, enzyme-linked and chemiluminescent immunoassays, and biomimetic immunosensor, with special emphasis on the challenges in developing biomimetic enzyme-linked immunosorbent assays (BELISAs). The biomimetic immunoassay method will provide an important new analysis platform in food safety, although the sensitivity and specificity is relatively low. Practical Application: As a new simple analysis method, the biomimetic immunoassay has attractive prospect, although some limitations were existed in real-sample assay. In this critical review, some promising solutions for overcoming its drawbacks were put forward, which may promote the more quick development and extensive application of this method in food safety
Template and target information: Review - MIPs in biomimetic immunoassays
Author keywords: application, artificial antibody, biomimetic immunoassay, molecular imprinting


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