MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Lv YK, Zhao MG, Zhang D, Yan HY
Article Title: Enrofloxacin-imprinted monolithic HPLC columns synthesized by in situ copolymerization for chromatographic separation.
Publication date: 2011
Journal: Journal of Liquid Chromatography & Related Technologies
Volume: 34
Issue: (9)
Page numbers: 705-718.
DOI: 10.1080/10826076.2011.562592
Alternative URL: http://www.informaworld.com/10.1080/10826076.2011.562592

Abstract: A monolithic molecularly imprinted polymer for enrofloxacin was synthesized by in situ copolymerization of methylacrylic acid and methyl methacrylate in a traditional stainless-steel chromatographic column for the rapid separation and determination of enrofloxacin and ciprofloxacin in eggs. By optimizing the polymerization condition, the monolithic MIP showed highly specific recognition for enrofloxacin over its active metabolite ciprofloxacin. The influence of components of monomers on hydrophobicity, contractibility, selectivity, and binding capacity of polymer were investigated. As shown by SEM, the mesopores and macropores were formed in the monolith column; this allowed the mobile phase to flow through the column with low backpressure. Furthermore, some chromatographic conditions such as mobile phase composition and flow rate on the retention times were investigated. The binding capacity was evaluated by static adsorption and Scatchard analysis, which showed that the dissociation constant and the maximum binding capacity were 117.7 mg/L and 27.27 mg/g for high affinity binding site, and 297 mg/L and 187.4 mg/g for lower affinity binding site, respectively. The mean recoveries of enrofloxacin and ciprofloxacin from egg samples were 103.3 - 115% and 89.3 - 95.1%, respectively. The research has shown that copolymerization provides more adjustable conditions for making molecularly imprinted monolithic columns with different morphologies and higher selectivity
Template and target information: enrofloxacin

  Live and Let MIP spoof movie poster bag  Chemistry peptide mug  Chemists are fun customisable shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner