Abstract: Molecular imprinting of peptides in aqueous media is always a challenge in the synthesis of artificial receptors. In this work, a new molecularly imprinted polymer (MIP) for thymopentin (TP-5) was prepared by the combined use of N-isopropylacrylamide (NIPAm) and N-t-butylacrylamide (TBAm) as functional monomers in the presence of N,N'-methylenebisacrylamide (BIS) as cross-linker, and the imprinted nanoparticles can be synthesized using a simple precipitation polymerization method. The preparation conditions were optimized by investigating the effect of the concentrations of the monomer and cross-linker on the adsorption properies. Dynamic light scattering (DLS) was used to investigate the dynamic process of particle growth. The resulting MIPs nanopartile were average diameter of 300 nm with the polydispersity (PDI) of 0.217. The adsorption properties of resultant imprinted polymers were evaluated by equilibrium rebinding experiments in an aqueous media. The highest binding capacity of TP-5 achieved from the optimized imprinted polymer was 194.72 μmol/g with an imprinting factor of 2.04. The results showed that the method established owns potential promises.
Template and target information: peptide, thymopentin, TP-5