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Abstract: A molecularly imprinted polymer was prepared by cyclic voltammetry method in gold electrodes using O-aminophenol as the functional monomer,melamine as the template,K3Fe(CN)6 as the imprinting electrode and the bottom of liquid probe,based on which a new method for detecting melamine rapidly and selectively in liquid milk was constructed.The optimum reaction conditions during the polymerization process were as follows,the ratio of monomers and templates of 0.275:1,pH 5.5, 30 °C,and elution time of 6min.The linear range of the method is 0.5~7.5mg/kg,the relative standard deviation is 6.1%~6.4%,the limit for determination is 0.5mg/kg,and the time for analysis is 6min.The recovery is 87.5%~109% when testing the actual liquid milk samples.This method could be used for detecting melamine in liquid milk continuously and automatically with high selectivity and sensitivity.
Template and target information: melamine
Author keywords: Melamine, molecular imprinting, electropolymerization, Electrochemical sensor