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Reference type: Journal
Authors: Yuan LH, Zhang J, Zhou P, Chen JX, Wang RY, Wen TT, Li Y, Zhou XM, Jiang HJ
Article Title: Electrochemical sensor based on molecularly imprinted membranes at platinum nanoparticles-modified electrode for determination of 17β-estradiol.
Publication date: 2011
Journal: Biosensors and Bioelectronics
Volume: 29
Issue: (1)
Page numbers: 29-33.
DOI: 10.1016/j.bios.2011.07.058
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566311005057

Abstract: In this paper, an electrochemical sensor for 17β-estradiol (E2) based on the molecular imprinting polymer (MIP) membranes had been constructed. 6-mercaptonicotinic acid (MNA) and E2 were first assembled on the surface of platinum nanoparticles-modified glassy carbon electrode (PtNPs/GCE) by the formation of Pt-S bonds and hydrogen-bonding interactions, and subsequently the polymer membranes were formed by electropolymerization. Finally, a novel molecularly imprinted sensor (MIS) was obtained after removal of E2. Experimental parameters such as deposition time, scan cycles, pH value and accumulation condition were optimized. Under optimal conditions, the MIS exhibited a large adsorption capacity and high selectivity. A good linearity was obtained in the range of 3.0 x 10-8 - 5.0 x 10-5 mol L-1 (r = 0.996) with an estimated detection limit of 1.6 x 10-8 mol L-1. MIS had been successfully used to analyze E2 in water samples without complex pretreatment. Meanwhile, the average recoveries were higher than 93.9% with RSD < 3.7%. All results above reveal that MIS is an effective electrochemical technique to determine E2 real-time in complicated matrix
Template and target information: 17β-estradiol, E2
Author keywords: 17β-estradiol, Molecularly imprinted polymers, Electrochemical sensor, water samples


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