MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: McNiven S, Kato M, Levi R, Yano K, Karube I
Article Title: Chloramphenicol sensor based on an in situ imprinted polymer.
Publication date: 1998
Journal: Analytica Chimica Acta
Volume: 365
Issue: (1-3)
Page numbers: 69-74.
DOI: 10.1016/S0003-2670(98)00096-8

Abstract: We have recently developed a liquid-chromatography (LC)-based sensor for the broad-spectrum antibiotic chloramphenicol which relies upon the displacement of a chloramphenicol (CAP) dye conjugate from a molecularly imprinted polymer by the drug. The sensor was immune to interference from various chloramphenicol derivatives and approximate to 60% as responsive to thiamphenicol (TAM), an almost identical analog. The system showed a linear response to the drug over the 3- 1000 mu g ml(-1) range; it was therefore effective both below and above the therapeutic (10-20 mu g/ml(-1) serum range, being potentially toxic above 25 mu g ml(-1)). The sensor effectively detected CAP extracted from serum with a 3 sigma detection limit of ca. 5 mu g ml(-1) We have further simplified the construction of the sensor by the use of an in-situ imprinting technique wherein the polymer is formed inside an LC column. These columns are far simpler to prepare and, although their characteristics are somewhat different, their performance is comparable with that of the bulk-polymerized materials. The capacity of the columns is significantly less than that of packed columns but their ability to separate TAM and CAP is slightly better. The best of these columns showed a fairly linear response (r(2)=0.95, n=3) for 0-30 mu g ml(-1) CAP with a detection limit of ca. 3 mu g ml(-1). The column also showed a linear (r(2)=0.96, n=3), although lesser response to TAM over a similar concentration range. As with the bulk-polymerized material, the system showed minimal response to the diacetyl derivative CAP-DA. (C) 1998 Elsevier Science B.V

  Periodic table Teacher shirt  PhD Scientist peptide mug  Gravity waves shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner