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Reference type: Journal
Authors: Kan XW, Xing ZL, Zhu AH, Zhao Z, Xu GL, Li C, Zhou H
Article Title: Molecularly imprinted polymers based electrochemical sensor for bovine hemoglobin recognition.
Publication date: 2012
Journal: Sensors and Actuators B: Chemical
Volume: 168
Issue: (1)
Page numbers: 395-401.
DOI: 10.1016/j.snb.2012.04.043
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0925400512003929

Abstract: A simple and efficient molecularly imprinted polymers (MIPs) based electrochemical sensor were proposed and prepared by electropolymerization of pyrrole in the presence of bovine hemoglobin (BHb) in an aqueous solution. The fabrication process of the sensor was characterized by differential pulse voltammetry and electrochemical impedance spectroscopy, in which [Fe(CN)6]3- /[Fe(CN)6]4- was used as an electrochemical active probe. The influence factors including electropolymerization cycles, scan rate, the concentration of pyrrole, and the extraction conditions were investigated in detailed. Under the optimized conditions, the experimental results showed that the MIPs based sensor possessed a fast rebinding dynamics and an excellent recognition capacity to BHb, compared to the other non-template proteins. Moreover, the prepared sensor also exhibited a dependent relationship between the concentration of template protein and peak current of [Fe(CN)6]3-/[Fe(CN)6]4-
Template and target information: protein, bovine serum albumin, BSA
Author keywords: Electrochemical sensor, Molecularly imprinted polymers, Pyrrole, Bovine hemoglobin, Recognition


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