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Reference type: Journal
Authors: Ying XG, Zhang FJ, Zhang LG, Cheng GX
Article Title: Preparation and Characterization of Protein Imprinted Calcium Alginate Microspheres Modified by Interpenetrating Networks.
Publication date: 2011
Journal: Journal of Tianjin University
Volume: 44
Issue: (2)
Page numbers: 134-140.
Alternative URL: http://en.cnki.com.cn/Article_en/CJFDTotal-TJDX201102008.htm

Abstract: In order to improve crosslinking strength and hydrogel stability,cellulose ether was introduced into cal-cium alginate and crosslinked to form interpenetrating networks(IPNs).Protein and emulsion dually imprinted cal-cium alginate microspheres were prepared with the modified hydrogel and were applied for selective rebinding.The microspheres were found to be more mechanically stable and more resisting to 0.9% sodium chloride aqueous solution,and the damage ratio was reduced from 24.4% to 9.84% according to oscillation test.The infrared spectra measurement indicated that new chemical bonds were formed between glutaraldehyde and cellulose,which con-structed the interpenetrating networks.Protein molecule imprinting and rebinding experiments were performed and imprinting efficiency was increased from 2.19 to 2.70 towards target molecular in the microspheres modified by IPNs.An improvement in separation factor from 1.478 to 18.88 was confirmed by ion-exchange chromatography experi-ments.The above results could be ascribed to the compact network structure due to the association of stronger covalent crosslinkages in the IPNs and the hydrophobic micro-environment.The IPNs enhance the imprint structure stability and increase rebinding specificity.
Template and target information: protein


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