Abstract: The molecularly imprinted polymer (MIP) has been prepared using levofloxacin and ciprofloxacin as the mixed-templates, a-methacrylic acid(MAA) as functional monomers and trimethylolpropane trimethylacrylate (TRIM) as crosslinker. A method has been established for simultaneous determination of residues of ten fluoroquinolones in grass carp by high performance liquid chromatography-ion trap mass spectrometry (HPLC-ITMS) combining with molecularly imprinted polymer solid phase extraction (MISPE). The samples were extracted with 2% acetic acid in acetonitrile and defatted with n-hexane, then purified by the self-made molecularly imprinted cartridge. The preparation of samples and the separation conditions had been optimized, HPLC was performed with a gradient elution program while acetonitrile and water (containing 0.05% formic acid) were used as mobile phase. The identification and quantification were done with ion trap mass spectrometry. The recoveries ranged from 80.6% to 104.6% with a relative standard deviations of 8.6%. The limits of detectio n(LOD) were 0.11-0.25 μg/kg and the limits of quantification (LOQ) were 0.35-0.84 μg/kg. This method is suitable for validation and multi-residue analysis of fluoroquolones in aquatic products.
Template and target information: fluoroquinolines
Author keywords: High performance liquid chromatography-ion trap mass spectrometry, Mixed-templates imprinted polymer, Fluoroquinolones