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Reference type: Journal
Authors: Baggiani C, Giraudi G, Trotta F, Giovannoli C, Vanni A
Article Title: Chromatographic characterization of a molecular imprinted polymer binding cortisol.
Publication date: 2000
Journal: Talanta
Volume: 51
Issue: (1)
Page numbers: 71-75.
DOI: 10.1016/S0039-9140(99)00251-9

Abstract: A cortisol-binding polymer was prepared by utilising a non-covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-keto- progesterone, 11 alpha-hydroxyprogesterone, 17 alpha- hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21- acetate with chloroform, containing 0.5% (v/v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11 alpha-hydroxyprogesterone, was found. (C) 2000 Elsevier Science B.V. All rights reserved
Template and target information: cortisol, cortisone, 11-α-hydroxyprogesterone

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