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Reference type: Journal
Authors: Ogawa KI, Hyuga M, Okada T, Minoura N
Article Title: Development of lipid A-imprinted polymer hydrogels that selectively recognize lipopolysaccharides.
Publication date: 2012
Journal: Biosensors and Bioelectronics
Volume: 38
Issue: (1)
Page numbers: 215-219.
DOI: 10.1016/j.bios.2012.05.028
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566312003405

Abstract: To remove lipopolysaccharide (LPS) from pure water, we developed polymer hydrogels that selectively recognize LPS. A molecular imprinting technique was used to prepare the polymer hydrogels. We prepared the polymer hydrogels with LPS-binding sites by using acryloyllysine and acryloylphenylalanine as functional monomers and used lipid A as a template because it is the biologically active part of LPS and contains two phosphate groups. Co-existence of n-octane during the polymerization process was highly effective in promoting the formation of LPS-accessible sites on the surface of the hydrogels. Both an electrostatic and a hydrophobic interaction between the lipid A portion of LPS and the recognition site of the imprinted hydrogel are necessary for LPS recognition. The adsorption isotherm of LPS to the lipid A-imprinted hydrogels was Langmuir-type; the saturated adsorption capacity and the adsorption constant, calculated by applying an equation for Langmuir-type adsorption isotherms, were 1.0 x 10-11 mol/cm2 and 2.5 x 10 5 M-1, respectively. The imprinted hydrogels selectively recognized toxic LPS in a competition experiment in which two other kinds of LPS with similar chemical structures to that of the LPS of E. coli (toxic LPS) were adsorbed to the lipid A-imprinted hydrogels
Template and target information: lipid A, lipopolysaccharide, LPS
Author keywords: Lipopolysaccharide, Lipid A, molecular imprinting, Polymer hydrogel, adsorption isotherm, molecular recognition

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