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Reference type: Journal
Authors: Zheng HF, Du XZ
Article Title: Reduced steric hindrance and optimized spatial arrangement of carbohydrate ligands in imprinted monolayers for enhanced protein binding.
Publication date: 2013
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes
Volume: 1828
Issue: (2)
Page numbers: 792-800.
DOI: 10.1016/j.bbamem.2012.11.006
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0005273612003847

Abstract: Imprinted monolayers provide several advantages over bulk imprinting methods. This is especially important for large templates such as proteins. Concanavalin A (Con A)-imprinted binary monolayers consisting of glycolipids with oligo(ethylene glycol) (OEG) spacers and zwitterionic phospholipids (DPPC) were constructed and investigated. The shorter phosphorylcholine (PC) headgroups with an almost flat-on orientation in the binary monolayers gave rise to reduced steric hindrance favorable to the accommodation of Con A with greater ease and facilitated the access of the OEG-linked mannose moieties for enhanced protein binding. Further enhanced binding resulted from optimized spatial rearrangement of the glycolipids at the air-water interface directed by Con A in the subphase to create bivalent binding sites and to minimize steric crowding of neighboring mannose ligands. The combination of the exposed carbohydrate ligands from biologically inert surfaces and the optimized ligand arrangement is the most reasonable solution to enhancement of protein affinity. The bivalent carbohydrate binding sites protruding from the imprinted monolayers were created to be complementary to the Con A binding pockets. This strategy generates tailor-made surfaces with enhanced protein binding and opens the possibility of controlled assembly of intellectual biomaterials and preparation of biosensors
Template and target information: protein, Concanavalin A, Con A
Author keywords: Lipid monolayer, Multivalent protein binding, protein imprinting, Infrared spectroscopy, surface plasmon resonance


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