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Reference type: Journal
Authors: Ohya Y, Miyaoka J, Ouchi T
Article Title: Recruitment of enzyme activity in albumin by molecular imprinting.
Publication date: 1996
Journal: Macromolecular Rapid Communications
Volume: 17
Issue: (12)
Page numbers: 871-874.
DOI: 10.1002/marc.1996.030171205

Abstract: The catalytic activity of bovine serum albumin (BSA) modified physically by molecular imprinting using transition-state analogue (TSA) as a template molecule was studied. The resultant imprinted serum albumin (Imp-BSA) showed the rate acceleration of dehydrofluorination reaction from (4R,4S)-4-fluoro-4-(4- nitrophenyl)butan-2-one (1) and followed the type of Michaelis-Menten reaction in ethyl acetate solution. The enzymatic activity of Imp-BSA was competitively inhibited by (4R,4S)-4-hydroxy-4-(4- nitrophenyl)butan-2-one (4)


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