MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Zhao DY, Qiao XG, Xu ZX, Xu R, Yan ZH
Article Title: Development of a biomimetic enzyme-linked immunosorbent assay method based on a hydrophilic molecularly imprinted polymer film for determination of olaquindox in chick feed samples.
Publication date: 2013
Journal: Journal of Immunoassay and Immunochemistry
Volume: 34
Issue: (1)
Page numbers: 16-29.
DOI: 10.1080/15321819.2012.668149

Abstract: In this study, a new way to substitute the biology antibody was introduced by using a hydrophilic molecularly imprinted film, which was directly prepared on the well surface of MaxiSorp polystyrene 96-well plate by the bulk polymerization technique. This imprinted film exhibited good recognition and fast adsorption-desorption dynamics toward olaquindox. Using it as the recognition element, a fast and new direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method for the determination of olaquindox in chick feed was developed. This BELISA method had low cross-reactivities of 6.2% and 12% for two analogues. Under the optimal conditions, the sensitivity (IC50) and the limit of detection (IC15) were 700 60 μg L-1 and 17.0 1.6 μg L-1, respectively. The blank chick feed samples spiked with olaquindox at three levels were determined by this developed method with recoveries ranging from 82.0 - 96.0%
Template and target information: olaquindox
Author keywords: artificial antibody, Biomimetic enzyme-linked immunosorbent assay, hydrophilic imprinted film, molecular imprinting technology

  mipdatabase.com logo special offer joke shirt  Lab Chick Periodic table shirt  HE-MAN polymer chemistry shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner