Abstract: A molecular imprinting electrochemical sensor for the determination of L-tryptophan was developed. The molecular imprinting polymer (MIP) was fabricated by the polymerization reaction of ethyl silicate (TEOS), phenyltrimethoxysilane (PTMOS) and phenyltrimethoxysilane (MTMOS). The presence and removal of the template molecule of L-tryptophan in the MIP film were confirmed by cyclic voltammetry. The sensor showed an excellent selectivity and a high sensitivity for L-tryptophan detection,with a linear range from 1.0 x 10-9 to 1.0 x 10-5 mol/L (r=0.9958) and a limit of detection as low as 1.0 x 10-12 mol/L. The sensor was successfully applied to the determination L-tryptophan in the serum samples and the recoveries were in the range of 80.0%~104%.
Template and target information: L-tryptophan
Author keywords: Electrochemical sensor, molecularly imprinted polymer, L-tryptophan