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Reference type: Journal
Authors: Pauling L, Campbell DH
Article Title: The manufacture of antibodies in vitro.
Publication date: 1942
Journal: Journal of Experimental Medicine
Volume: 76
Page numbers: 211-220.

Abstract: A protein solution with the properties of a specific antiserum to the triphenylmethane dye methyl blue has been made by treating a solution of bovine g-globulin and the dye with alkali and then slowly neutralizing the alkali. Some success has been obtained also in the formation of antibodies from other serum proteins and by other denaturation-renaturation procedures.By heating solutions of g-globulin and antigen to 57C. for several days antisera homologous to the antigens have been prepared. This method has been used successfully with the azodye 1,3-dihydroxy-2,4,6-tri(p-azophenyl-arsonic acid) benzene and with pneumococcus polysaccharide Type III. The antipneumococcus sera were found to precipitate the polysaccharide of Type III but not those of Types I and VIII and to agglutinate pneumococci of Type III but not those of Types I and II.


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