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Reference type: Journal
Authors: Yang T, Wu YL, Li C, Zhao J, Chen G, Lü Y
Article Title: Preparation and Characterization of Ractopamine-Imprinted Material Using Surface-Molecular Imprinting Method and Its Adsorption Characteristics.
Publication date: 2013
Journal: Scientia Agricultura Sinica
Volume: 46
Issue: (6)
Page numbers: 1256-1262.
DOI: 10.3864/j.issn.0578-1752.2013.06.020
Alternative URL:

Abstract: [Objective] The objective of this experiment is to prepare a molecule-imprinted material on surface of silica gel for ractopamine. [Method] Polymethylacrylic acid (PMAA) was grafted onto the surface of silica gel particles via the coupling effect of γ-methacryloxypropyl trimethoxy silane,and the grafting particle PMAA/SiO2 was prepared. Then, the molecular imprinting towards the grafted PMAA was performed with ractopamine as template molecule and ethylene glycol diglycidylether (EGDE) as crosslinking agent by using the surface-molecular imprinting technique established, and the ractopamine molecule-imprinted material MIP-PMAA/SiO2 was prepared. The morphology of MIP-PMAA/SiO2 was observed by SEM, and the chemical structure of MIP-PMAA/SiO2 was characterized by FT-IR. Static method was adopted to study the binding properties and molecule recognition characters of MIP-PMAA/SiO2 for ractopamine. [Result] The MIP-PMAA/SiO2 on the adsorption ability of ractopamine was significantly better than clenbuterol and salbutamol. The adsorption capacity of the MIP-PMAA/SiO2 was 11.08 mg g-1, the specificity binding capacity was 9.93 mg g-1, and the imprinted factor of the polymer was 9.63. The adsorption reaction reached equilibrium after 10 min, and the desorption ration of ractopamine reached 92.3% in 5 min. [Conclusion] The experimental results show that MIP-PMAA/SiO2 have specific recognition selectivity, excellent binding affinity and elution property for ractopamine.
Template and target information: ractopamine
Author keywords: surface molecular imprinting, Ractopamine, characterization, Adsorption characteristics

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