MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Gao FX, Ma XT, He XW, Li WY, Zhang YK
Article Title: Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein.
Publication date: 2013
Journal: Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume: 433
Page numbers: 191-199.
DOI: 10.1016/j.colsurfa.2013.05.018
Alternative URL: http://www.sciencedirect.com/science/article/pii/S092777571300397X

Abstract: Through covalent immobilization of template and surface imprinting, smart molecularly imprinted polymer nanospheres were developed for selective separation of the glycoprotein ovalbumin (OB). First, a boronic acid group-bearing poly (methyl methacrylate) (b-PMMA) nanosphere was synthesized directly at the high temperature of 70 C. The b-PMMA nanosphere could pre-immobilize the template OB on its surface by forming reversible covalent bonds. Then the precipitation polymerization of N-isopropylacrylamide (NIPAAm) and acrylamide (AAm) readily occurred on the b-PMMA nanosphere as a core at room temperature, leading to the formation of core-shell molecular imprinting nanosphere. The experiments showed the rebinding affinity of the imprinted nanospheres was thermo- and pH-dependent. The resulting imprinted nanospheres showed high adsorption capacity and good specific recognition behavior toward the template molecule, and no obvious reusability deterioration was observed. Most notably, the imprinted nanospheres reached saturated adsorption within 20 min, indicating faster rebinding kinetics. In addition, the imprinted nanospheres were successfully applied to selectively separate the target OB from an egg white sample
Template and target information: protein, ovalbumin, OB
Author keywords: molecular imprinting, surface imprinting, covalent immobilization, Nanosphere, Ovalbumin

  Periodic table Coach hat  Life Science peptide mug  We need to talk about Kelvin shirt

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner