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Reference type: Journal
Authors: Saridakis E, Chayen NE
Article Title: Imprinted polymers assisting protein crystallization.
Publication date: 2013
Journal: Trends In Biotechnology
Volume: 31
Issue: (9)
Page numbers: 515-520.
DOI: 10.1016/j.tibtech.2013.05.003

Abstract: Molecularly imprinted polymers (MIPs) are 'smart materials' polymerised in the presence of a template molecule, of which they retain a chemical 'memory'. When the template molecule is extracted from the polymer, it leaves behind cavities that are complementary to it, thus making the material capable of rebinding that molecule with high affinity and selectivity. Such materials, imprinted both with small molecule and with protein templates, have been used in chromatographic, chemical, and biological sensing applications. Here, we review a variety of uses for MIPs, focusing on their recently discovered role as nucleation inducing substances for protein crystals. This discovery makes them useful tailor-made 'nucleants' to be used both for optimisation of protein crystal growth and for discovering new crystallization conditions
Template and target information: Review - MIPs as nucleants in crystallisation
Author keywords: Molecularly imprinted polymers, polymers, protein crystallization, nucleation, nucleants

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