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Reference type: Journal
Authors: da Silva H, Pacheco JG, Magalh„es JMCS, Viswanathan S, Delerue-Matos C
Article Title: MIP-graphene-modified glassy carbon electrode for the determination of trimethoprim.
Publication date: 2014
Journal: Biosensors and Bioelectronics
Volume: 52
Page numbers: 56-61.
DOI: 10.1016/j.bios.2013.08.035
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566313005848

Abstract: A novel sensitive electrochemical sensor was developed by electropolymerization of pyrrole (PY) and molecularly imprinted polymer (MIP) which was synthesized onto a glassy carbon electrode (GCE) in aqueous solution using cyclic voltammetry in the presence of Trimethoprim (TMP) as template molecules. Furthermore, a previous electrode modification was performed by deposition of a suspension of graphene on the electrode's surface. The performance of the imprinted and non-imprinted (NIP) films was evaluated by impedance spectroscopy (EIS) and cyclic voltammetry (CV) of a ferric solution. The molecularly imprinted film exhibited a high selectivity and sensitivity toward TMP. The sensor presented a linear range, between peak current intensity and logarithm of TMP concentration between 1.0 x 10-6 and 1.0 x 10-4 M. The results were accurate (with recoveries higher than 94%), precise (with standard deviations less than 5%) and the detection limit was 1.3 x 10-7 M. The new sensor is selective, simple to construct and easy to operate. The MIP sensor was successfully applied to quantify TMP in urine samples
Template and target information: trimethoprim, TMP
Author keywords: trimethoprim, Electrochemical sensor, Molecularly Imprinting Polymer (MIP), Graphene, Pyrrole


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