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Reference type: Journal
Authors: Prasad BB, Srivastava A, Prasad A, Tiwari MP
Article Title: Molecularly imprinted micro solid-phase extraction technique coupled with complementary molecularly imprinted polymer-sensor for ultra trace analysis of epinephrine in real samples.
Publication date: 2014
Journal: Colloids and Surfaces B: Biointerfaces
Volume: 113
Page numbers: 69-76.
DOI: 10.1016/j.colsurfb.2013.08.046
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0927776513005638

Abstract: A simple hyphenation approach was adopted to obtain a new molecularly imprinted micro solid-phase extraction fiber (as a selective extraction tool) and complementary molecularly imprinted polymer coated pencil graphite electrode (as a detection tool) for the selective and sensitive analysis of epinephrine, which is a disease biomarker prevalent at ultra trace level in biological fluids. In both extraction and detection processes, the functionalized multiwalled carbon nanotubes (CNT-mers) were preferred to multiwalled carbon nanotubes (unmodified) in order to obtain a stable homogeneously dispersed imprinted polymer matrix of better electroconductivity and adsorptive characteristics. The hyphenation of both tools helped dual pre-concentration of epinephrine so as to achieve the stringent limit [limit of detection: 0.002 ng mL-1, S/N = 3] of clinical detection, without any problems of non-specific contributions and cross-reactivity
Template and target information: epinephrine, adrenaline
Author keywords: Molecularly imprinted micro solid-phase extraction, epinephrine, Differential pulse anodic stripping voltammetry, CNT-mer, Real sample analysis


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