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Reference type: Journal
Authors: Yang YQ, He XW, Wang YZ, Li WY, Zhang YK
Article Title: Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin.
Publication date: 2014
Journal: Biosensors and Bioelectronics
Volume: 54
Page numbers: 266-272.
DOI: 10.1016/j.bios.2013.11.004
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0956566313007811

Abstract: A novel epitope molecularly imprinted polymer (EMIP) for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin (BSA) was demonstrated where polymerization was performed on the surface of silica nanospheres embedded CdTe quantum dots (QDs). The synthetic peptide derived from the surface-exposed C-terminus of bovine serum albumin (BSA, residues 599-607) was selected as the template molecule. The resulting EMIP film was able to selectively capture the template peptide and the corresponding target protein BSA via the recognition cavities. Based on the fluorescence quenching, the EMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using epitope as the template) nanospheres were successfully applied to the direct fluorescence quantification of BSA. Compared with BMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using BSA as the template), the imprinting factor and adsorption capacity of EMIP-coated QDs were greatly increased. The prepared EMIP-coated QDs can also discriminate even one mismatched sequences from the original sequences of the epitope of the BSA. The practical analytical performance of the EMIP-coated QDs was examined by evaluating the detection of BSA in the bovine calf serum sample with satisfactory results. In addition, the resulting EMIP-coated QDs nanospheres were also successfully applied to separating BSA from the bovine blood sample
Template and target information: protein, peptide, epitope, bovine serum albumin, BSA, surface-exposed C-terminus of bovine serum albumin, BSA, residues 599-607
Author keywords: Epitope imprinting, Bovine serum albumin, Quantum dots, Fluorescent quantification


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