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Reference type: Journal
Authors: Zhong S, Kong YY, Zhou L, Zhou CY, Zhang XN, Wang Y
Article Title: Efficient conversion of myricetin from Ampelopsis grossedentata extracts and its purification by MIP-SPE.
Publication date: 2014
Journal: Journal of Chromatography B
Volume: 945-946
Page numbers: 39-45.
DOI: 10.1016/j.jchromb.2013.11.036
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1570023213006491

Abstract: In this study, we developed an efficient conversion process of dihydromyricetin to myricetin from Ampelopsis grossedentata extracts. The content of myricetin increased from 2.38% to 85.57%, demonstrating the successful dehydrogenation of dihydromyricetin. Molecularly imprinted polymers (MIPs) were prepared by surface imprinting method using silica microspheres as the support matrices and myricetin as template. The MIPs were applied for the selective adsorption of myricetin. The chemical structure of the MIPs was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. Static, dynamic and selective adsorption experiments showed that the MIPs exhibited good adsorption ability, rather fast template rebinding kinetics, and appreciate selectivity over structurally related compounds. Accordingly, the MIPs were applied as the selective sorbent in SPE to purify myricetin obtained through dehydrogenation, followed by HPLC-UV analysis. The recoveries of myricetin and dihydromyricetin were 92.7% and 55.6%, respectively. This study demonstrates the feasibility of using the developed MIP-SPE method to purify and enrich myricetin in the natural products
Template and target information: myricetin
Author keywords: Ampelopsis grossedentata, Dehydrogenation, molecularly imprinted microspheres, Myricetin, SPE


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