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Reference type: Journal
Authors: Liu R, Sha M, Jiang SS, Luo J, Liu XY
Article Title: A facile approach for imprinting protein on the surface of multi-walled carbon nanotubes.
Publication date: 2014
Journal: Talanta
Volume: 120
Page numbers: 76-83.
DOI: 10.1016/j.talanta.2013.12.002
Alternative URL: http://www.sciencedirect.com/science/article/pii/S003991401300982X

Abstract: This study describes a green, facile and low cost approach for imprinting protein on the surface of multi-walled carbon nanotubes (MWNTs) using papain as the template, dopamine as the functional monomer. By simply mixing MWNTs, dopamine, template protein in weak alkaline aqueous solution, a thin adherent polydopamine (PDA) film imprinted with protein was spontaneously obtained on the surface of MWNTs to produce the imprinted nanomaterials (MWNTs@MIPs). The obtained MWNTs@MIPs were characterized with Fourier transform infrared spectrometer (FT-IR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The adsorption process of the MWNTs@MIPs towards template protein was investigated in detail. The effects of the concentration of the monomer and template, polymerization time, extraction process were optimized. The prepared MWNTs@MIPs show fast binding kinetics, high binding capacity and acceptable specific recognition behavior towards template proteins. Furthermore, the stability and regeneration were also investigated, which indicated that the MWNTs@MIPs had good reusability. The good recognizing behavior coupled to the low cost and facile one-step preparation make the MWNTs@MIPs attractive for separation and specific protein recognition
Template and target information: protein, papain
Author keywords: molecular imprinting, MWNTs, protein, Polydopamine


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