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Reference type: Book Chapter
Authors: Hoshino F, Watanabe O, Wu XH, Takimoto Y, Osawa T
Publication date: 2014
Chapter title: Low-Cost and Easy-to-Use "on-Chip ELISA" for Developing Health-Promoting Foods.
Page numbers: 151-161.
DOI: 10.1007/978-94-007-7920-4_13

Book title: Lipid Hydroperoxide-Derived Modification of Biomolecules
Edition: 77
Editors: Kato Y
Publisher: Springer Netherlands
City: Dordrecht
ISBN: 978-94-007-7919-8
Series title: Subcellular Biochemistry

Abstract: We have determined that a biological molecule can be physically immobilized on a polymer containing an azobenzene (azopolymer) using irradiating light. We immobilized antibodies and antigens on the surface of an azopolymer coated glass slide (antibody array) to establish "on-chip ELISAs". The assays used the flat-surface of a glass slide and could be applied to both sandwich and competitive ELISAs. The sensitivity and accuracy of the on-chip ELISA were similar to a conventional ELISA using a polystyrene plate. Using the assay system, we proved that representative oxidative-biomarkers could be simultaneously measured from μL of urine. That should realize low-cost study on animal or human, and accelerate development of health-promoting foods. So, this new concept antibody array has promising applications in proteomic studies, and could be used to examine biomarkers to investigate health-promoting food
Author keywords: antibody, ELISA, Hexanoyl lysine, 8-OHdG

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