MIPs logo MIPdatabase    HoHoHo MIP2018 Conference website
Custom Search
Reference type: Book Chapter
Authors: Hoshino F, Watanabe O, Wu XH, Takimoto Y, Osawa T
Publication date: 2014
Chapter title: Low-Cost and Easy-to-Use "on-Chip ELISA" for Developing Health-Promoting Foods.
Page numbers: 151-161.
DOI: 10.1007/978-94-007-7920-4_13

Book title: Lipid Hydroperoxide-Derived Modification of Biomolecules
Edition: 77
Editors: Kato Y
Publisher: Springer Netherlands
City: Dordrecht
ISBN: 978-94-007-7919-8
Series title: Subcellular Biochemistry

Abstract: We have determined that a biological molecule can be physically immobilized on a polymer containing an azobenzene (azopolymer) using irradiating light. We immobilized antibodies and antigens on the surface of an azopolymer coated glass slide (antibody array) to establish "on-chip ELISAs". The assays used the flat-surface of a glass slide and could be applied to both sandwich and competitive ELISAs. The sensitivity and accuracy of the on-chip ELISA were similar to a conventional ELISA using a polystyrene plate. Using the assay system, we proved that representative oxidative-biomarkers could be simultaneously measured from μL of urine. That should realize low-cost study on animal or human, and accelerate development of health-promoting foods. So, this new concept antibody array has promising applications in proteomic studies, and could be used to examine biomarkers to investigate health-promoting food
Author keywords: antibody, ELISA, Hexanoyl lysine, 8-OHdG


  I love MIPs bag  multi MIPs logo mug  American periodic table patriotic shirt

Molecules Special Issue call     Sensors Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon