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Abstract: This is the first reported work of artificial α-amanitin receptors which are prepared using the design and synthesis of a template molecule based on an α-amanitin recognition determinant imprinting strategy. The resultant molecularly imprinted polymers (MIPs) are evaluated using high performance liquid chromatography (HPLC), binding experiment, nitrogen adsorption measurement, and solid-phase extraction. Experiment clearly demonstrates that the MIPs as the HPLC stationary phase can specifically recognize α-amanitin from analogues. The MIPs also successfully adsorb trace amounts of α-amanitin in spiked serum samples selectively pretreated and enriched through molecularly imprinted solid phase extraction. The limit of detection and recovery is 3.0 ng mL-1 and 88.5-95.9%, respectively, for α-amanitin in serum samples. The high specific adsorption and excellent selectivity of the MIPs arises from imprinting effects related to the imprinting cavity of the polymeric matrix, the metal-coordination bond, and the hydrogen bond between the receptor and ligand
Template and target information: α-amanitin
Author keywords: α-Amanitin, molecular imprinted polymers, high performance liquid chromatography, Solid-phase extraction