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Reference type: Journal
Authors: Zhang M, Wang YZ, Jia XP, He MZ, Xu ML, Yang S, Zhang CJ
Article Title: The preparation of magnetic molecularly imprinted nanoparticles for the recognition of bovine hemoglobin.
Publication date: 2014
Journal: Talanta
Volume: 120
Page numbers: 376-385.
DOI: 10.1016/j.talanta.2013.12.004
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0039914013009867

Abstract: The protein imprinted technique combining surface imprinting and nano-sized supports materials is an attractive strategy for protein recognition and rapid separation. In this work, we imprinted bovine hemoglobin (BHb) on magnetic nanoparticles. With itaconic acid (IA) and acrylamide (AAm) as the monomers, the experiment was carried out in aqueous media via surface-imprinting technique. The effects of initial concentration and adsorption time over the adsorption capacity of both imprinted and non-imprinted nanoparticles were analyzed. The maximum adsorption capability of imprinted nanoparticles was found to be 77.6 mg g-1, which was 3.1-4.3 times higher than that of the non-imprinted nanoparticles prepared at the same conditions. This resulted in the successful formation of imprinting cavities. Moreover, in selective adsorption experiment and competitive batch rebinding test, imprinted nanoparticles exhibited a high specific recognition of the template protein over the non-imprinted protein
Template and target information: protein, bovine hemoglobin, BHb
Author keywords: surface imprinting, magnetic nanoparticles, Itaconic acid, Bovine hemoglobin


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