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Reference type: Journal
Authors: Zhu QF, Ma C, Chen HX, Wu YQ, Huang JL
Article Title: A molecular imprint-coated stirrer bar for selective extraction of caffeine, theobromine and theophylline.
Publication date: 2014
Journal: Microchimica Acta
Volume: 181
Issue: (3-4)
Page numbers: 303-311.
DOI: 10.1007/s00604-013-1117-1

Abstract: We have prepared a novel caffeine imprinted polymer on a stir bar that can be used for selective extraction of caffeine, theobromine and theophylline from beverages. The polymerization time and quantities of reagents (template, cross-linker, porogenic solvent) were optimized. The morphology of the molecularly imprinted polymer-coating was studied by scanning electron microscopy and Fourier transform IR spectroscopy. A rapid and sensitive method was worked out for the extraction of caffeine, theobromine and theophylline from beverages by using the molecularly imprinted stir bar followed by HPLC analysis. The effects of extraction solvent, stirring speed, desorption solvent, adsorption and desorption time were optimized. The method displays a linear response in the 5-150 μg L-1 caffein concentration range, with a correlation coefficient of > 0.9904. The recoveries for three analytes in tea, carbonated and functional beverages were 91-108 %, 90-110 % and 93-109 %, with relative standard deviations ranging from 3.6-5.7 %, 3.5-7.9 % and 3.2-7.9 %, respectively
Template and target information: caffeine, theobromine, theophylline
Author keywords: molecularly imprinted polymer, Stirrer bar sorptive extraction, caffeine, Beverages


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