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Reference type: Journal
Authors: Aswini KK, Mohan AMV, Biju VM
Article Title: Molecularly imprinted polymer based electrochemical detection of L-cysteine at carbon paste electrode.
Publication date: 2014
Journal: Materials Science and Engineering: C
Volume: 37
Page numbers: 321-326.
DOI: 10.1016/j.msec.2014.01.020
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0928493114000289

Abstract: A methacrylic acid (MAA) based molecularly imprinted polymer (MIP) modified carbon paste electrode (CPE) was developed for electrochemical detection of L-cysteine (Cys). Characterisation of MIP was done with FTIR and the modified electrode with cyclic voltammetry (CV) and differential pulse voltammetry (DPV). CV, DPV and impedance analysis demonstrated that the modified electrode is responsive towards the target molecule. The optimum percentage composition of MIP for MIP/CPE and the effect of pH towards the electrode response for Cys were studied. The detection of Cys in the range of 2 x 10-8 to 18 x 10-8 M at MIP/CPE was monitored by DPV with a limit of detection of 9.6 nM and R2 of 0.9974. Also, various physiological interferents such as ascorbic acid, L-tryptophan, D-glucose, D-cysteine and L-cysteine were found to have little effect on DPV response at MIP/CPE. The utility of the electrode was proved by the effective detection of Cys from tap water and human blood plasma samples with reproducible results
Template and target information: L-cysteine
Author keywords: molecular imprinted polymer, L-cysteine, carbon paste electrode, Ferro-ferricyanide


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