Inventors: Chang J, He RQ, Jun Z, Zhang LP, Meng ZH, Luan YJ, Hao HX, Yu ZS, He Y
Patent Title: Brodifacoum (anticoagulation raticide) molecularly imprinted polymer and use thereof of as solid phase extraction agent
Publication date: 2011
Publication number: CN102070753

Abstract: The invention relates to a brodifacoum (anticoagulation raticide) molecularly imprinted polymer and the use thereof of as a solid phase extraction agent and belongs to the technical field of analytical chemistry and material science. Brodifacoum molecules serving as template molecules and a functional monomer are uniformly mixed in a solvent and then stored at a temperature below 4 DEG C for 24 hours to form a prepolymer; adding a cross-linking agent and an initiator at normal temperature, and initiating polymerization for 24 hours by using ultraviolet light with a wavelength of 260 nanometer to obtain spherical particle polymer; and soaking the obtained spherical particle polymer in methanol/acetic acid serving as an organic solvent, washing to remove impurities, drying under vacuum, and obtaining the brodifacoum molecularly imprinted polymer. In the invention, the preparation method is simple, convenient and quick; thebrodifacoum molecularly imprinted polymer has high analogue (such as brodifacoum and bromadiolone) absorption performance; and the established solid-phase extraction method is simple in operation, absorbs target materials selectively, removes impurities from a substrate effectively, improves separation precision, lowers detection limits, and is suitable for extracting and separating brodifacoum and bromadiolone anticoagulation raticides from a biological sample (blood) in the field of forensic science.
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