Abstract: BACKGROUND: Binary functional monomers, allyl-β-cyclodextrin (allyl-β-CD) and methacrylic acid (MAA) or allyl-β-CD and acrylonitrile (AN), were exploited in a fabrication of molecularly imprinted polymers (MIPs) for selective recognition and large enrichment of pirimicarb from aqueous media. RESULTS: Special attention was paid to the computational simulation of the inprinting molecular and functional monomers. The morphological characteristics of MIPs made of allyl-β-CD and MAA (M-MAA) were characterized by a scanning electron micrograph (SEM). The effect of binding capacity of MAA-linked allyl-β-CD MIPs (M-MAA) demonstrated higher efficiency than that of AN-linked allyl-β-CD MIPs (M-AN) when tested in binding specificity. Finally, M-MAA was chosen to run through molecularly imprinted solid-phase extraction (MISPE) to analyze the spiked fresh leafy vegetables of pirimicarb. CONCLUSION: The present proposed technique is a promising tool for the preparation of the receptors which could recognize pirimicarb pesticide in aqueous media
Author keywords: Molecularly-imprinted polymer (MIP), Molecularly imprinted solid-phase extraction (MISPE), Pirimicarb, Simulation, cyclodextrin