Abstract: For the first time a molecularly imprinted polymer based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms which has a high homology to the human enzyme. The template was removed either by treatment with proteinase K or by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine based MIP-sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection 3.97 nM (R2 = 0.994) and shows good discrimination towards other proteins, e.g. bovine serum albumin and cytochrome c.
Author keywords: Molecularly imprinted polymers, biomimetic sensors, Tyrosinase, Electropolymerization, Scopoletin, o-Phenylenediamine