Abstract: A simple, rapid, low cost and selective magnetic solid phase microextraction method using magnetic molecularly imprinted polymer (MMIP) combined with chemiluminescence (CL) was developed for the separation/preconcentration and determination of furazolidone (FZD) antibiotic. The sorbent was synthesized using bulk polymerization method. The SiO2-coated Fe3O4 nanoparticles were used as the magnetic supporter, FZD as the template, 4-vinylpyridine as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the crosslinker and toluene as the porogen solvent. The synthesized MMIP was characterized by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) spectroscopy, and vibrating sample magnetometer (VSM) analysis. The MMIP nanoparticles showed sorption capacity as high as 49.6 mg g-1. Under the optimized conditions, the calibration graph was linear (R2 = 0.9991) over the range of 0.1-10.0 μg L-1 with the limit of detection of 0.027 μg L-1 for the preconcentration of 100 mL of the sample and 0.5 mL of eluent. The relative standard deviation (RSD%) at the 1.0 μg L-1 level of FZD (n = 6) for intera- and inter-day analysis were 4.7 and 5.4%, respectively. The developed method was successfully applied to the determination of trace amounts of FZD in poultry tissues and water samples