Abstract: A novel molecular imprinting polymers (MIPs)-based electrochemical senors, consisting of Fe3O4 nanobeads and gold nanoparticles loaded on the reduced graphene oxide (RGO) substrate, are fabricated to detect ractopamine (RAC) in water using reversible addition fragmentation chain transfer (RAFT) polymerization technique. Au nanoparticles are widely dispersed on RGO via energy dispersive spectrometer (EDS) mapping rather than on Fe3O4 nanobeads. The Au nanoparticles on graphene can significantly increase the response current for detecting RAC in water by means of enhancing the electroconductivity of the composite, which is confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and theoretical calculation. As such, the hybrid electrode shows a dynamic linear range of 0 to 0.1 μM with a correlation coefficient of 0.992 and a remarkably low detection limit of 1.745 x 10-11 mol L-1 (S/N = 3). Additionally, the sensor exhibits high binding affinity and selectivity towards RAC with excellent reproducibility and stability. Our study demonstrates the potential for the proposed electrochemical sensors in monitoring organic pollutant in water