Abstract: The preparation of raw materials is an important prerequisite to study the function of natural active ingredients. Here, the preparation of the MWCNTs/ZIF-67@MIPs based on surface molecular imprinting technology (SMIT) for the separation of bilobalide (BB) from real samples was proposed. The successful preparation of the MWCNTs/ZIF-67@MIPs was demonstrated by SEM, FTIR, XRD and TGA. The adsorption equilibrium was achieved when the time was 4 h, and the rate of adsorption calculated from the dynamic adsorption results was 14.2 μg/mL/h. The maximum adsorption amount for MWCNTs/ZIF-67@MIPs to BB was calculated to be 59.2 mg/g, which was more than three times that for the MWCNTs/ZIF-67@NIPs (23.1 mg/g), and the Freundlich isotherm model and pseudo-second-order kinetic model were more suitable for the explanation of isothermal and kinetic adsorption behaviors, respectively. The results of selective adsorption indicated that the MWCNTs/ZIF-67@MIPs show excellent selectivity and specificity for BB compared to similar structural compounds such as GA, GB and GC with an excellent imprinting factor (IF=2.83). The results of reuse studies indicated that MWCNTs/ZIF-67@MIPs exhibited great reproducibility with the recovery rate of 70.8% after repeated use for 5 times. Finally, the developed MWCNTs/ZIF-67@MIPs were used as solid phase extraction (SPE ) sorbent to directly separate BB from the treated Gingko leaves extract and crude extract of BB, and the results suggested that the purity of BB in the two samples was increased by about 30%, and the recoveries reached 68.34% and 71.54%, respectively.Meanwhile, the separation effect of MWCNTs/ZIF-67@MIPs on BB was better than that of BBMIP prepared by bulk polymerization. This article is protected by copyright. All rights reserved
Author keywords: Bilobalide, surface molecular imprinting, MWCNTs, ZIF-67 composites, Adsorption, Solid phase extraction