Abstract: Nowadays, molecularly imprinted polymers (MIPs) coated silica stationary phases (SPs) have aroused great attention, owing to their good properties of high selectivity, good stability, facile synthesis procedure and low cost. In this study, zidovudine imprinted polymers coated silica stationary phases (MIPs/SiO2 SPs) were synthesized by surface imprinting technique using zidovudine as the template molecule, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linking agent, azobisisobutyronitrile as the initiator, and bare silica spheres (particle size, 5 μm; pore size, 20 nm) as substrates. In the process, reagents with low concentration were used to prepare thin layer of MIPs coating on the surface of silica microbeads. The properties of the materials were characterized by scanning electron microscope (SEM), fourier transform infrared spectrometer (FT-IR), carbon elemental analysis and N2 adsorption-desorption experiment. The obtained SPs were packed into stainless steel columns (2.1 mm × 150 mm) via a slurry method. The prepared columns were applied for separation of nucleoside analogues with similar chemical structures and strong polarity. The retention mechanism of MIPs/SiO2 SPs for nucleoside analogues was investigated carefully. And the chromatographic performances of the resulting MIPs based SPs were superior to those of the commercial SPs. Furthermore, the synthesized MIPs/SiO2 SPs possessed great potentials in separation of ginsenosides. This investigation demonstrated that MIPs based SPs were successfully synthesized and provided a new approach to polar compounds separation and analysis
Author keywords: Molecularly imprinted polymers (MIPs), High performance liquid chromatography, Stationary phases, Silica microbeads, Nucleoside analogues