Abstract: A novel molecularly imprinted electrochemical sensor based on ferric oxide nanoparticles modified glassy carbon electrode (GCE) was fabricated for detection of human growth hormone (HGH). The stable proteins molecularly imprinted polymer (MIP) films were made by electro-polymerization using aniline as functional monomer and HGH as the template. Electropolymerization was done in a range of - 0.3 to + 0.9 V at a scan rate of 0.1 V s-1 for 15 cycles. The accuracy of MIP/Fe3O4/GCE formation was confirmed using the square wave voltammetry technique from - 0.2 to + 0.6 V, in 5 mM solution of [Fe(CN)6]3-/4- with 0.1 M KCl. According to the results, the sensor showed a linear range from 1.0 × 10-10 to 1.0 × 10-7 g/cm3 of HGH (R2 = 0.9588) under optimal conditions with a detection limit of 0.6 × 10-10 g/cm3. The calibration curve showed a 95.8% correlation of the data. The human plasma sample analysis confirmed the applicability of the MIP/Fe3O4/GCE sensor to the assay of the HGH molecules. In conclusion, the sensor has sensitivity, high stability, and good repeatability
Author keywords: Molecularly Imprinted Electrochemical Sensor, Growth hormone, Fe3O4 nanoparticles, aniline, Square wave voltammetry